Genetic variation among three population for Old World Screwworm Chrysomya bezziana (Diptera: Caliphoridae) in Northern, Middle and Southern of Iraq by using RAPD-PCR technique

Ahmed Sultan AL-Qeraqouly, Ammar

	Genetic variation among three population for Old World Screwworm Chrysomya bezziana (Diptera: Caliphoridae) in Northern, Middle and Southern of Iraq by using RAPD-PCR technique
Diyala Journal For Pure Science
Article 1, Volume 13, Issue 3, 2017, Pages 176-191
Author
Ammar Ahmed Sultan AL-Qeraqouly
Abstract
Randomly amplified polymorphic DNA polymerase chain reaction RAPD-PCR technique was used in this study to investigate genetic relationship among three populations of Old World Screwworm Chrysomya bezziana from Northern, Middle and Southern Iraq. Environmental conditions such as temperature, relative humidity and the amount of annual rainfall to be different in the northern, Middle and Southern Iraq , In addition to the northern of Iraq away from the southern a distance of 1,000 km and away from the middle distance of approximately 500 km or less, as well as there are some mountains and rivers separating northern Iraq from the middle and south , these environmental conditions act as geographical barriers to gen flow among the three population. We have used in this study seven primers and Tools For Population Genetic Analysis (TFPGA) software was used to calculate percentage efficiency of each primer and percentage of discriminatory ability of each primer , In addition to Nei’s genetic identity. The results showed that the primers No. 3 , 4 and 6 gives The highest number of polymorphism and highest of percentage efficiency and percentage of discriminatory ability which is 7, 20 and 21.2 respectively ., While primer No. 2 gave The lowest of polymorphism and percentage efficiency and percentage of discriminatory ability which is 2 , 5.71 and 6.06 respectively ., When comparing among three population through genetic identity observed that Ch.bezziana of northern Iraq close genetically to Ch.bezziana of middle Iraq, the degree of genetic convergence is 0.111. While Ch.bezziana of northern Iraq far from the genetically Ch.bezziana from southern Iraq, the degree of genetic convergence is 0.264 . While they are close genetically to Ch.bezziana of middle Iraq the degree of genetic convergence is 0.127 . which reveals that there is a low level of genetic identity reflecting the fact that the environmental conditions appear to act as a barrier to gene flow among these populations.
Keywords
Chrysomya bezziana; RAPD; PCR; Southern; Middle; northern Iraq

Statistics Article View: 79 PDF Download: 51