Investigation on bla Ctx-M-1, Virulence Factors and Multidruge Resistance in Pseudomonas Aeruginosa Bacteria

Rahman Rasheed Al-Taai, Hadi; Mohammed Hameed, Zainab; Mohammed Jasim, Izdehar

	Investigation on bla Ctx-M-1, Virulence Factors and Multidruge Resistance in Pseudomonas Aeruginosa Bacteria
Diyala Journal For Pure Science
Article 1, Volume 13, Issue 3, 2017, Pages 207-222
Authors
Hadi Rahman Rasheed Al-Taai; Zainab Mohammed Hameed; Izdehar Mohammed Jasim
Abstract
One hundred seventy six clinical specimens were included in this study. These specimens included burns, wounds, ear and urinary tract infections from different ages and sex and from resident and out patients in the hospital . Twenty isolates of P. aeruginosa were recovered (11.36%) after do bactereiological and biochemical tests by using VITEK 2 compact .The antibiotic susceptibility patterns for 8 antibiotics were carried, the antibiotic resistance of P. aeruginosa isolates to cephalosporines was appeared to be variable, so that the resistant rate to Ceftazidime, Cefatoxime, , Cephalothin and Cephalexin were 90%, 25%, 100% and 100% respectively. The minimum inhibitory concentrations (MIC) of the Cefotaxime and Ceftrazidime were determined by serial double dilutions technique The MIC for Cefatoxime was 8-512, and for Ceftazidime was 8-1024. All P. aeruginosa isolates were investigated for detection of broad spectrum β-lactamases, 55% of the isolates were appeared to have the ability for broad spectrum β-lactamases. The detection of the presence of bla CTX-M-1 was done , the isolates possession rate for bla CTX-M-1 was 72.7%. Furthermore , the production of virulence factors which include the ability for the biofilms formation and the detection of efflux pumps were done , 100% of the isolates were biofilms former, and 95% of the isolates possess the efflux pumps.
Keywords
aeruginosa; MDR; bla CTX; blaCTX

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