(2014). Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals. , 10(3), 307-315.
. "Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals". , 10, 3, 2014, 307-315.
(2014). 'Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals', , 10(3), pp. 307-315.
Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals. , 2014; 10(3): 307-315.

Clinical Isolation and Molecular Diagnosis of Cutaneous Leishmaniasis by using KDNA Gene PCR at some Baghdad Hospitals

journal of kerbala university
Article 1, Volume 10, Issue 3, 2014, Pages 307-315    PDF (0 K)
Abstract
This study was aimed to find out the most effective clinical samples and diagnosis methods in chronic cutaneous leishmaniasis (CCL). Smear, aspiration fluid, and filter paper, samples were taken from 44 skin lesions of suspected cases with CCL in some Baghdad hospitals, and they were compared using microscopic examination, culture, and molecular methods.Samples from the clinical patients were examid which showed throug PCR 95.45%(42/44) ,Microscopic examination 72.7%(32/44), culture 27%(12/44).During the study ,products in KDNA –PCR, L.tropica and L. major the observation of expected bands 780 and 650bp,and the filter paper most effective clinical samples from lesion.In conclusion the PCR –based assays tested on our increased the speed and sensitivity of the diagnosis DNA –PCR very important to the species identification ( L. tropica and L. major).
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