Evaluation of Efficiency Genetic Mutations at Cellulomonas gelida In Stimullating of Cellulase System

.A . R.Al-Nashe, Ali

	Evaluation of Efficiency Genetic Mutations at Cellulomonas gelida In Stimullating of Cellulase System
journal of al-qadisiyah for pure science(quarterly)
Article 1, Volume 6, Issue 1, 2006, Pages 201-215 PDF (0 K)
Author
Ali .A . R.Al-Nashe
Abstract
Summary The aim of this study was evaluation of efficiency of some mulation strains Cellulomonas gelida in their cellulase activity in solid and lequid cellulose media .These mutations were stimulated by chemical and physical technique. The mutational strains of this bacteria appeared best cellulase activity compared with original bacteria . On solid cellulose medium the cellulase activity has been cleary through meserment of radial of soft region that empty of cellulose were been in 4th days of incubation: (24 , 27,23,27,30,26) mm .For (MCA1,MCA2,MCA3,MCB1,MCB2,MCB3) respectively.Where the redial of this region was 18 mm in original strain .It was seemed the cellulase activity of these mutation strains in lequid cellulose medium were different behavior and types , also different in periods of appearing with differents of these stains The strains (MCB2 and MCA2) had been higher Endoglucanas activity 6.3 and 6.6 (IU) in 2nd and 3rd days respectively. The strains (MCB1 and MCA3) had been high Exoglucanase activity 0.5 and 0.55 (IU) in 2nd and 3th day respectively. The strains (MCA3 and MCA2) had been higher B-glucosidase activity (0.82 and 0.97)IU in 2nd and 3th days respectivily The study appeared that Endoglucanase and Exoglucanase were Exocellular enzymes glucosidase found cell wall linked enzyme or -which found free in culture while Bendocellular enzyme .

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