استخلاص إنزيم اليوريز وتصنيعه محليا من بذور فول الصويا لغرض استخدامه في تحليل يوريا الدم

	استخلاص إنزيم اليوريز وتصنيعه محليا من بذور فول الصويا لغرض استخدامه في تحليل يوريا الدم
journal of al-qadisiyah for pure science(quarterly)
Article 1, Volume 6, Issue 4, 2006, Pages 179-187 PDF (0 K)
Abstract
Abstract This study tackled on of the important scientific & economic topics whic is the process of extracting the urease enzyme from the soybean seeds whic is grown widely in Iraq particulary in wassit goverment ( mainly in Al Dalmaj & Al Dujaili areas ) its also aimed at using this enzyme in measuring the rates of blood urea and urine urea of human beings . Since Iraq depends on urease enzyme which is imported and extracted from ( Jackbean ) and due to the permanent need of it in all labs of biochemical analysis in Iraq , So this enzyme had been extracted according to scientific procedure and made in lab kit which satisfied the need of the whole country 1. The soybean was taken from local market . 2. It was cleaned well from imperfeetions and extraneous substances . 3. Soaked for 15 minutes to get rid of husks . 4. Dried under 25 c for 24 hours . 5. grinded by an electrical grinder (whic care the temperature) . 6. Seived out by a sieve of holes with 425 mm diameter . 7. Acetone 99% was used to get rid of the oil in the amount of 4 part acetone to 1 part powder . 8. Magnetic blender was used in lab temperature to mix under 21 – 25 c for 15 minutes . 9. The residue was separated from the acetone and the dissolved oil by the cooled centrifugation . 10. The residue whic contained the enzyme was drid grinded saftenes by a glass mortar and then packed in small bottles with sccure caps 11 kept in a cool dry place for use the method of nessler was used in measuring the blood urea to extract healthy & patients ascending weights were used it was 10 , 20 , 30 , 40 , 50 , 60 ,70 .

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