Morphological and Molecular Identification of Mycorrhizal Fungi Infection for Several Plants Grown in Gypsiferous Soil

H. Altai, Salahuldeen; E. Alkurtany, Abedulkareem; M. Jasim, Hameed

	Morphological and Molecular Identification of Mycorrhizal Fungi Infection for Several Plants Grown in Gypsiferous Soil
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 17, Issue 3, 2017, Pages 193-201
Authors
Salahuldeen H. Altai; Abedulkareem E. Alkurtany; Hameed M. Jasim
Abstract
A field survey was conducted in Tikrit University, Salah Al-din governorate to Identify Mycorrhizal Fungi for grown Plants in College of Agriculture on 2014, The plant samples are collected randomly from 6 Locations different in there Texture , Chemical , physical and Fertilize Characteristics , included (10) different plant families (Corn- Zea mays , Barley- Hordeum vulgare, Wheat- Triticum aestivum , Onion- Allium cepa , Bean- Vicia faba , Peas- Lathyrus odoratusL., Chick-peas- Cicer Linnaeus , Alfalfa- Medicago truncatula , Spotted medic- Melilotus officinalis , - Nigella sativa ) the total number of samples (25) contained root plant and there surrounding soil (Rhizosphere) The results showed :The highest Percentage of VAM infection presente in Corn and Onion which was 60% for each one , while the less Percentage of VAM infection presence in Spotted medic , Alfalfa , it was 22.5 , 15% respectively,The result of gel electrophoreses proved that the Extraction DNA method was very appropriate to get the concentration and purity for amplification gene with PCR technique ,.The result showed that the nucleus primer NS1 was very accurate to Identify the Mycorrhizal fungi infection in the plant root and different between the infected and non-infected root.
Keywords
Morphological and Molecular Identification; Mycorrhizal Fungi Infection; plants; Gypsiferous soil; PCR

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