Molecular Study of Aeromonasspp. Isolated from Clinical and Environmental Samples

M. Salman, Safaa

	Molecular Study of Aeromonasspp. Isolated from Clinical and Environmental Samples
journal of al-qadisiyah for pure science(quarterly)
Article 1, Volume 15, Issue 2, 2015, Pages 45-55 PDF (0 K)
Author
Safaa M. Salman
Abstract
Receved :7/4/2015 Accepted : 9/6/2015 and Azhar N. Hussien AL-Qadisiya University / Collegeof Education / Biology Department E-Mails: Abstract A total of 259 specimen from different clinical sources from patients of AL-Diwanyia's hospitals and environmental sources ( water) were collected. Biochemical and morphological characterization tests besides the use of API 20 E and Vitek System and Polymerase Chain Reaction (PCR)Technique which used 16S rDNA geneshowed that seventeen isolates were identified as Aeromonas spp. These Isolates distributed as : 8 isolates belong to A. hydrophila , 4 isolates belong to A. sobria , 4 isolates belong to A. caviae and one isolate belong to A. salmonicida . PCR was used for detecting virulence factors such as exotoxin genes : hemolysin (hly A) , aerolysin(aer A) and cytolytic enterotoxin (aer) , results showed that 77.7% and 62.5% of environmental and clinical isolates had hly A while 44.4 % and 50 % 54 2015 ﺔﻨﺳ 2 ﺩﺪﻌﻟﺍ 20 ﺪﻠﺠﻤﻟﺍ ﺔﻓﺮﺼﻟﺍ ﻡﻮﻠﻌﻠﻟ ﺔﻴﺳﺩﺎﻘﻟﺍ ﺔﻠﺠﻣ had aer A , aer gene just found in clinical isolates in rate 25% while didn't found in environmental isolates .The same technique (PCR)was also used for detecting resistance genes of beta - lactam antibiotic : blaCMY , blaTEM , blaCTX-M , and blaSHV genes which used in this study . The results showed that 11.76% had blaCMYgene , 5.88% had blaTEM gene and 17.64% had blaCTX-M gene of all isolates , while blaSHV gene didn't found in all the types of isolates which isolated from clinical and environmental sources .
Keywords
Aeromonas; Aerolysin; Hemolysin; Cytolytic enterotoxin

Statistics Article View: 184 PDF Download: 66