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Antifungal activity of agent produced by Streptomycesspp. isolated from soil samples | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 15, Issue 2, 2015, Pages 30-36 PDF (0 K) | ||
| Author | ||
| Samer M. Al-Hulu | ||
| Abstract | ||
| Thirty soil samples were collected from Hilla City. Thirteen actinomycetes isolates was isolated. Antifungal activity of these isolates were tested against C. albicans . Results showed that actinomycetes. 4 was given higher inhibition zone compared with other isolates. Actinomycetes. 4 isolate was selected for extraction of antifungal agent. According to cultural and biochemical tests of Actinomyces. 4 these isolates was belong to Streptomyces spp. Streptomyces spp.4 isolate was grey aerial mycelium, and yellow-green substrate mycelium , no melanin production and no diffusible pigment, able to ferment sugars, negative for catalase and H2S and positive for citrate utilization. Crude extract of Streptomyces spp.4 was active against C.albicans and A.niger with inhibition zone (22mm) against C. albicans and (16 mm) against A. niger. MIC of Streptomyces spp.4 antifungal agent showed that MIC values against C. albicans , A. niger were 22, 38 µg/ ml respectively. UV spectrum of absorption for antifungal agent showed that it have a single peak with maximum absorption (λ max) 293 nm. | ||
| Keywords | ||
| Streptomyces; antifungal activity; extraction of antifungal agent | ||
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