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Study of Ossification Stages in Quail Embryo (Coturnex japonica) | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 15, Issue 2, 2015, Pages 44-52 PDF (0 K) | ||
| Author | ||
| Hashim M.A-Kareem | ||
| Abstract | ||
| A morphogenesis study has been made for the skeleton formation of the quail (Coturnex japonica ) which included the analytical study to make the skeletal elements and the transitional changes that happens in the two processes of cartilage formation (Chondrofication ) and bones (Ossification) during the growth of the different natural phases for the diverse bones of the body. A quail was gathered of about (100) birds and brought up at home. Then the fertilized egg was gathered from those birds in about (7-9) days, those egg were incubated by an Ukranian electronic incubator in a about ( 37,5-38 Co) and 70% of temperature and humidity respectively. The embryos were used starting from day 3 of incubation to day 16 of incubation , which is the hatching day. Embryos were extracted from the egg starting from day 3 of incubation,they were treated with alcian blue pigment that is related to paint the (non ossified) cartilaginous parts of bones, and alizarin red – s pigment that is related to paint the ossified parts of the bones as an indicator for calcium in order to recognize the ossified parts from those that didn't ossified for each bone of the skeletal system of the quail.The method that used to treat the embryos with the two pigments differed with the age progress of the embryo , also it's concentration increased with the progress of the embryos age due to the formation of the organs, skin and muscles as well as the strengthening of the cartilage and it's transformation into solid bones day after day. The successive phases for the growth of the different bones were traced with complete concentration on the evolution of the cartilage (Chondrofication) and it's transitional transformation into bone (Ossification) for each bone of the vertebral skeleton , thorax gage , and the fore and hind limbs bones. | ||
| Keywords | ||
| Qiail Bird; fertilizedegg; Quail emboryo and growth | ||
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