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Evaluation of Candida albicans Diagnosis by using conventional PCR | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 12, Issue 1, 2012, Pages 1-6 PDF (0 K) | ||
| Author | ||
| Nihad A.M. Al-Rashedi | ||
| Abstract | ||
| This study involved evaluate conventional polymerase chain reaction (PCR) technique using kit 500/730 IC(Sacace, Italy) to detect Candida albicans in urine of female patients from Samawah gynaecological hospital. The evaluation performed by comparing C. albicans PCR direct diagnosis of clinical specimens with germ tube formation and api Candida system (bioMe´rieux, France). We collected 116 urine specimens of female patients in age ranging 20-50 years. The results of DNA amplification tests show 50 of these 116 specimens were positive for 500 b.p band on agarose gel. Both specificity and sensitivity of PCR diagnosis of Candida albicans were 100%. | ||
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