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Study on effect of Vitamin C and E (normal and double dose) on skin wounds healing in experimental rats wistar albino | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 12, Issue 2, 2012, Pages 33-54 PDF (0 K) | ||
| Authors | ||
| Shada M.AL-Sudani; Maha K.AL-Mallak | ||
| Abstract | ||
| This present study was investigated the effect of certain nourishing factors such as vitamin C and E (normal and double dose) on skin wounds healing in Laboratory rats. Total number of animals were (90) divided into (3) groups, with (30) rats in each. All animals were wounded on dorsal back within an area of (4cm2). Animals of fist group (GI) were treated after wounded intraperitoneally with 250mg ⁄ kg of vitamin C while control animals in this group treated with 0.01ml ⁄ gm of distilled water, where’s the animals of second and third groups (Gll,Glll)covered daily with normal dose of vitamin E(30mg ⁄ kg) and double dose (60mg ⁄ kg) respectively , while the wounds of control animals in these two groups covered with Vaseline only.Macroscopically changes accompanying wounds healing in both treated and control animals were daily recorded, also histological changes like average of inflammatory cells(Neutrophils and macrophages), Number of Fibroblasts, adipocytes density in addition to angiogenesis during healing was evaluated.Results clarified the effect of nourishing factors on collagen deposition , regeneration of skin layers and hair follicles appearance during periods (2,4,8,12,15,21) days post-wounding. Wounds treated with vitamin C and normal dose of vitamin E showed early healing signs represented by sever hyperemia, redness, dryness of wounds edges and formation of scar tissue without scars and traces compared to control animals. The study referred to an inhibition effect of double dose of vitamin E as wounds kept partially opened till the (15th) day with formation of scars and traces in the positions of wounds. Data also shown an increase with significant difference (P<0.05)in rate of neutrophils and macrophages during (2nd) and (4th) days post-wounding in animals treated with vitamin C and double dose of vitamin E compared to animals treated with normal dose of vitamin E and control animals. Proliferation of inflammatory cells gradually decreased in all wounds except that treated with double dose of vitamin E, which existence of neutrophils cells continuated in wounds. The study elaborated as well as the effect of nourishing factors on regeneration of epidermis and dermis layers within the wounds of animals treated compared to control animals. The histological sections shown complete regeneration of both treated wounds and control occurs on 21 days post-wounding. Also the results of histological investigation stressed the role of these factors in the appearance of hair follicles, vitamin C and normal dose of vitamin E have positive role in the early appearance of hair vesicles during (4th) days post-wounding compare with wounds treated with double dose of vitamin E which hair follicles appeared till the (12th) day and control wounds on eight day. 1-Introduction | ||
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