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Effect of Sodium Chloride ( NaCl ) and Biofertilizer (Agrispoon) on Growth of Catharanthus roseus (L.) G. Don and Production of Alkaloids Vincristine and Vinblastine | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 12, Issue 2, 2012, Pages 32-43 PDF (0 K) | ||
| Authors | ||
| Abdulameer Ali Yaseen; Layth Sareea Al-Rekaby | ||
| Abstract | ||
| This experiment was conducted in Biology Department of College of Science , Al-Qadisiya University during the period from 1/11/2009 to 1/4/2010. The goal of the experiment was to find the effect of four levels of sodium chloride include 0 and 6 and 9 and 12 dsm/m and three levels of biofertilizer (Agrispoon) include 0 and 3 and 6 ml/L on growth characteristics and production of medicinal active substance ( vincristine and vinblastine ) of Catharanthus roseus ( L.) G. Don . Forty eight transplants at age of one month were transplanted in plastic pots with dimension of 15 × 20 cm (one transplant per pot) in 1/11/2009 . The Agrispoon was sprayed twice during the growing season on 1/11/200 and 1/12/2009 at the early morning on the plant shoot till complete wetness . Sodium chloride were imposed on plants when even irrigation was needed .The design of the experiment was Randomized Complete Blocks Design (RCBD) in a factorial arrangement (4×3) in four replications . The Revised Least Significant Difference ( RLSD ) was used to compare treatment means at 5 % probability level when treatment effect was significant . Results were as follow : 1-Increasing sodium chloride concentration caused a significant decrease in leaf area average and dry weight of shoot , leaves content of total chlorophyll and nutrient elements N and K/Na ratio and caused a significant increased in leaf content of proline and VIC and VIB alkaloids . 2-Increasing biofertilizer concentration caused a significant increase in all vegetative growth characteristics except that of content of proline which was significantly decreased . 3- The interaction between sodium chloride and biofertilizer caused a significant increase in leaves content of VIC and VIB alkaloids . The highest amount of VIC was 0.3400 µgm/gm dry weight of leaves and VIB 0.6829 µgm/gm dry weight of leaves were produced from the combination treatment of 12 dsm/m salt and 6 ml/L biofertilizer . | ||
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