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Isolation and Studying of Cholera phage in Al-Diwanyia city and possibility of using it to control Vibrio cholerae phenotype 01 in laboratory | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 11, Issue 4, 2011, Pages 1-13 PDF (0 K) | ||
| Author | ||
| Ziad M. Al-khozai | ||
| Abstract | ||
| This work was carried out in the period between June 2009 to November 2010, water samples were collected from five positions of the river of Al-Diwanyia city, 50 sample were collected (25 from river and 25 from sewage and heavy water) to isolate bacteria and 45 sample were collected for isolation of phages (25 from river and 20 from sewage and heavy water) . Results showed that only three samples gave positive record for cholera from river about 12%, while sewage samples recorded 12 (48%) . Vibriophages were isolated from river about 56±22 pfu/ml while from sewage were 23±12 pfu/ml. Results also showed that the isolated vibriophages appeared at the maximum activity in the alkaline medium between 8-10 this activity was declined after 10, activity also was reduced in the acidic medium and activity end point was around 6. In 40Cº the phage activity was not affected but it was decreased continuously when temperature increase 50 Cº and 60 Cº, in 70 Cº phage activity was diminished in about 7-8 minutes. Highest adsorption rate of isolated vibriophages on bacterial cells was about 2-4 minutes, time increment significantly increase the adsorption while dilution and shaking reduce adsorption. Maximum lysis activity of bacterial cells was documented in about two hours and resulted in elevation of viral concentration and decreasing bacterial cell concentration in the same time, complete lysis time was about 6 hours. Results also showed that the sensitivity of isolated bacteria towards the phages was high especially to river phages about 88.5% and less to sewage phages 77.22%, while highest sensitivity was recorded in the positive reference strain 91%.Over all results of this work reflect the important role of vibriophages in controlling the levels of Vibrio cholerae in environment. | ||
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