Detection of some humoral autoimmune parameters in diabetes mellitus patients at Najaf city

A.H. AL-lybawy, Ali

	Detection of some humoral autoimmune parameters in diabetes mellitus patients at Najaf city
journal of al-qadisiyah for pure science(quarterly)
Article 1, Volume 11, Issue 4, 2011, Pages 22-32 PDF (0 K)
Author
Ali A.H. AL-lybawy
Abstract
An immunologic study were performed to determine the prevalence of autoantibodies to self-antigens in the cells of the pancreas, the two antibodies, glutamic acid decarboxylase autoantibodies( GADAs ) and islet cell autoantibodies ( ICAs ), as well as autoantibodies of the pituitary gland in the sera of patients with diabetes type I and for different age groups and both sexes of the patients for the period from January 2009 to June 2010. The patients and control groups were testing the proportion of sugar glucose in the blood before breakfast as well as checking all sera for the detection of glutamic acid decarboxylase autoantibodies by use enzyme linked immuno sorbent assay , as well as revealing islet cell autoantibodies and the pituitary gland autoantibodies for using indirect immunofluorescence assay, in addition to measured the concentrations of immunoglobulins IgG, IgA and IgM by single radial immuno diffusion assay . The study showed the spread of a high percentage of glutamic acid decarboxylase and islet cell autoantibodies in patients with type I diabetes , the percentage reached 48% and 56% respectively, while there have been no rate of those autoantibodies in apparently normal subjects and the differences were statically non significant below the level of ( P > 0.05 ) for these antibodies in both sexes. The rate of GADAs in females raised to 55.56 % while the rate in males reached 41.026 %, and the ICA was higher in males 58.97 % compared with their proportion in females 52.78 % as well as the proportion of those antibodies were decrease with progression age. The study showed that there is a weak link but without significant differences at ( P > 0.05 ) between the autoantibodies of the pituitary gland and type I diabetes mellitus , where found that the antibodies in sera of four patients only compared to the total number ( 75 patients ). The results also it was show high mean of concentrations of antibodies IgG, IgA and IgM in sera of patient's in comparison with the control group, also noted a decrease in concentrations of the immunoglobulins with the aged patients.

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