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Effect of phospholipase and the fungus which it produced (Aspergillus niger) on the some of blood parameters of male mice (Mus musculus) | ||
| journal of al-qadisiyah for pure science(quarterly) | ||
| Article 1, Volume 9, Issue 1, 2009, Pages 192-210 PDF (0 K) | ||
| Author | ||
| Mohammed A. Auda | ||
| Abstract | ||
| The present study included the isolation and identification of phospholipase from Aspergillus niger , and then the study of the effect of phospholipase and the fungus which it produced (Aspergillus niger) on some blood parameters of male mice. A.niger was isolated from patients with epidermal infection , and then growth on SDA media . The ability of fungus for product of phospholipase was examined by plate assay method under standard condition . The phospholipase was isolated after the growth of fungus on liquid media. The male mice were injected ( I. P. ) with 0.1 , 0.2 ml / animal /day for 7 days (first stage) and 14 days (second stage) from phospholipase enzyme and then it’s effect on some blood parameters were recorded. Other groups of male mice were injected with 0.1 and 0.2 ml /animal with suspension of A.niger spores for one time , then after 7 days (first stage) and 14 days (second stage) the some blood parameters were examined . The results indicated that the phospholipase and suspension of A.niger spores caused a significant reduction in blood parameters ( R.B.C. , P.C.V. , Hb and W.B.C. ) of male mice . | ||
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