Molecular Detection of Chlamydia Trachomatis In Women with Bad Obstetric History

Salmaan Hassan, Jabbar; Jabbar Al-Tamimi, Bushra; Ahmed Al-Hamawandi, Jwan

	Molecular Detection of Chlamydia Trachomatis In Women with Bad Obstetric History
Medical Journal of Babylon
Article 1, Volume 14, Issue 2, 2017, Pages 233-239
Authors
Jabbar Salmaan Hassan; Bushra Jabbar Al-Tamimi; Jwan Ahmed Al-Hamawandi
Abstract
Bad obstetric history is multifactorial common female genital disorders. A significant percentage of cases are attributed to infectious agents, of which Chlamydia trachomatis attracted less attention. This study aimed to assess the role of Chlamydia trachomatis in a sample of Iraqi women with bad obstetric history using molecular methods. This case-control study recruited 100 women with bad obstetric history (referred as cases onward) and 40 age-matched apparently healthy women as controls. Bacterial DNA was isolated from homogenized placental tissues obtained from each participant post-delivery. The 16S ribosomal gene of the Chlamydia trachomatis was amplified with specific set of primers using conventional PCR. Twenty-Three placental samples (23%) gave positive results for gene amplification from cases, while none of women in control groups gave such a result. The infection rates were significantly higher in 31-39 age group, aborted women in the first trimester and in women with more than 4 pregnancies compared to other age groups, women aborted in the trimester, and women with 4 or less pregnancies respectively. These data highly suggested the association of C. trachomatis with bad obstetric history. Screening program for detection of these bacteria in pregnant women should be considered.
Keywords
Chlamydia trachomatis; Bad obstetric history; PCR

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