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Lipase from S. aureus, Purification and Application of Three Characterizing Experiments | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 6, Issue 9, 2015, Pages 6-15 PDF (0 K) | ||
| Authors | ||
| Amir Hani Raziq; Asia Fadhil Redha; Rana A. Hanoon | ||
| Abstract | ||
| Many lipases have been extensively purified and characterized in terms of their activity and stability profiles relative to pH, temperature, and their effects as metal ions and chelating agents. One hundred samples were collected from different body sites and lesions of in and out patients who attented Al-Kadhumiyah and Baghdad Teaching Hospitals. Identification of bacterial isolates was performed by following the procedures mentioned elsewhere in the literature. Purification steps of lipase included: extraction of the enzyme, precipitation of the enzyme by ammonium sulphate, dialysis, ion-exchange chromatography, and gel filtration chromatography. The results revealed that the enzyme was purified with a yield of 0.3 and a fold of purification of 153.7. Also, the optimum temperature and pH for lipase stability were 40 0C and pH 8, respectively, while the molecular weight of the enzyme was 110000 daltons. | ||
| Keywords | ||
| Staphylococcus aureus; Lipase; Enzyme stability | ||
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