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Identification of Lipase ProducersStaphylococcus aureus and Studyingthe Effects of Some Physicochemical Factors on Lipase Production | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 6, Issue 9, 2015, Pages 30-38 PDF (0 K) | ||
| Authors | ||
| Amir Hani Raziq; Rana A. Hanoon; Asia Fadhil Redha | ||
| Abstract | ||
| Lipases catalyze both the hydrolysis and synthesis of triacylglycerols. In addition to the industrial uses of lipases, there is an evolving literature on their role as important microbial virulence factors. One hundred samples were collected from different body sites and lesions of in and out patients from both sexes who attended Al-Kadhimyia Teaching Hospital and Medical Laboratories in Baghdad Teaching Hospital during the period from March, 2012 until September, 2012 for the isolation and identification of Staphylococcus aureus. Identification of bacterial isolates was performed according to the standard protocols. According to lipase activity measurement, it was revealed that isolate no. 12 was the higher producer as compared to the others and thus it was selected for further work.The results demonstrated that the best lipase production was in the alkaline pH (8) and at temperature of 30oC. It is also shown that the addition of 0.001M BaCl2 and 2mM KCl to the culture media has dramatically increased lipase activity. Conversely, the addition of 10mM NaCl, 0.001M FeSO4.7H2O, and 2M NaF has caused significant reduction in lipase activity. | ||
| Keywords | ||
| Staphylococcus aureus; Lipase; Inorganic ions; Enzyme activity | ||
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