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Optimisation of Standard PCR Programme | ||
| karbala journal of pharmaceutical sciences | ||
| Article 1, Volume 6, Issue 10, 2015, Pages 0-0 PDF (0 K) | ||
| Author | ||
| Zaid Al-Obaidi | ||
| Abstract | ||
| PCR is a very sensitive technique that gained its significance through the high contribution on a variety of bioscience fields. It is not surprising that the most cited scientific paper in biosciences is related to the PCR programme. The optimisation of the PCR is very important to achieve amplification with reliable results. Variables like Mg++ concentration, template DNA dilution, annealing temperature, and primers' concentration were optimized using classical and touchdown amplification methods. Other PCR methods like hot-start can improve the quality and the quantity of the PCR yield. The utilization of mathematical and statistical ways in designing a PCR programme like Taguchi or chessboarding methods can efficiently enhance the PCR reaction. Also the usage of very pure reagents and the utilization of a suitable kind of the Taq polymerase would highly improve future experiments. This work was performed to find the optimal reaction conditions for the utilized standard PCR programme. | ||
| Keywords | ||
| Standard PCR Programme; concentration; Taq polymerase | ||
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