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Field Survey to Isolation and Morphological Identification of Mycorrhizal Fungi Spores and Use PCR Technique from Different Location in Tikrit University | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 17, Issue 4, 2017, Pages 190-203 | ||
| Authors | ||
| Salahuldeen H. Altai; Hameed M. J; Abedulkareem E. Alkurtany | ||
| Abstract | ||
| A field survey was conducted in Tikrit University - Salah Al-din governorate to Isolate and Identify Mycorrhizal Fungi spores from 6 Location different in Soil Texture , Chemical , physical and Fertility Characteristics , in College of Agriculture in 2014, included (10) different plants families, the total number of samples was (25) contained plant root and there surrounding soil (Rhizosphere) the Genera and Species of mycorrhizal fungi Isolated from all studied regions were (Glomus mossea , G. intradeces , Gigaspora sp. , Entrophospora sp., Acoulospora sp.) The results shown:- That Glomus genus was the most common in all of the studied regions and the second Genus Entrophospora sp. and thence Acoulospora sp., while Gigaspora sp. was the less common, The maximum spores account of mycorrhizal fungi in sandy loam soil surrounding Onion plants , and then Sand Soil (less and high gypsiferous) surrounding Corn plants , while the minimum spores recorded in the Loamy sand Soil surrounding Alfalfa plants ,The result of gel electrophoreses proved that the Extraction DNA method was very appropriate to get the concentration and purity for amplification gene with PCR technique, The result of gel electrophoreses with primer AML1 showed that we can differential between Mycorrhizal fungi Genera by PCR technique. | ||
| Keywords | ||
| Field survey; isolation; Morphological Identification; Mycorrhizal fungi spores; PCR technique; PCR; Glomus | ||
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