Resistance Klebsiella pneumoniae bacteria for some antibiotics isolated from Al-Rumetha city Hospital in  Al-Muthanaa Province

Mohameed Ali, Abeer

	Resistance Klebsiella pneumoniae bacteria for some antibiotics isolated from Al-Rumetha city Hospital in Al-Muthanaa Province
Journal of College of Education for Pure Science
Article 1, Volume 7, Issue 2, 2017, Pages 201-213
Author
Abeer Mohameed ali
Abstract
In this current study, collected 90 clinical samples from general Al-Rumetha city Hospital in Al-Muthanaa Province during period from August to October 2016 , clinical samples were included;30 urine samples, 30 wound swaps , 7 vagina swaps and 23 blood samples, then subjected to cultural and biochemical tests for the purpose of diagnosisKlebsiella pneumoniae bacteria, and then tested against 18 antimicrobial agent and found that 37(41.1%) isolatewas resistant to cefoxitin as follows ; 13(35.1%) isolates from urine , 10(27%) isolates from blood, 10(27%) isolates from wounds and 4(10.8%) isolates from vagina , so as the cefoxitin resistance isolates subjected to Modified three dimention test and AmpC disk testas well as the previous two testes result were positive for all isolates37(100%) . It has also been investigating AmpC- β-lactamasesbyPCR technique , found that 30(33.3%) isolates showed that positive result for theblaAmpCgene after that the isolates examined by using Multiplex PCR technique for the detection of blaACCand blaMox genes . The results of Multiplex PCR were as follows ; 14(46.7%) isolate contained blaMox gene and 2(6.7%) isolate contained blaACC gene, While the remaining 14(46.7%) isolates containing other genes such as blaDHA,blaFox, blaEBCand blaCIT as follows ; 7(23.3%) , 4(13.3%), 1(3.3%) and 2(6.7%) respectively.
Keywords
Klebsiella pneumoniae; blaACC; AmpC; lactamase

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