A Genetic  Polymorphism  of  Interleukin-1a and Interleukin 1 Receptor Antagonist Gene with type 1 Diabetes Mellitus of Iraqi  Patients

Badday Hassan, Ibtesam; Falh,; Maha,; Nazzal,

	A Genetic Polymorphism of Interleukin-1a and Interleukin 1 Receptor Antagonist Gene with type 1 Diabetes Mellitus of Iraqi Patients
Al-Ma'mon College Journal
Article 1, Volume 0, Issue 30, 2018, Pages 232-244
Authors
Ibtesam Badday Hassan; ; ;
Abstract
The aim of this study was to evaluate the frequency of polymorphism of interleukin-1-a gene (IL1a) at position-889 and IL-1 Receptor Antagonist Gene at position-maspl 11100 of the promoter region (IL1-a-889 and IL-1Rα-maspl 11100 SNP) in T1DM and in healthy controls subjects in thirty nine of Iraqi patients, (12 males & 27 females;1 5.65 ± 1.79 years) and 21 controls.(7 males & 14females ; 14.66 ± 3.43 years) were enrolled in this study the polymorphism of IL1-a-889 and IL1Ra-maspl 11100 were data waved by polymerase chain reaction-specific sequence primer (PCR-SSP) assay. Results revealed that comparing IL1a-889 genotypes and alleles between T1DM patients and controls frequencies of TT genotype and T allele (51.28 vs. 67.95%; P =0.511 respectively) were significantly high in patients in contrast to controls, (33.33 vs. 40.48%; P =0.179) and the related RR rates were 18.4 and 19.8, respectively, and the associated EF values were 1.51 and 1.82 . In contrast CC genotype and C allele (15.38 vs. 32.05 %, P =0.264 respectively) frequencies were significantly decreased in patients, compared to controls (52.38 vs. 59.52%; P =0.179), and associated PF values were 0.73 and 0.66 , respectively. But for IL-1Rα-maspl 11100 polymorphism frequencies of TT genotype and T allele (35.89 vs. 42.31%; P=0.125) were significantly decreased in patients compared to controls (47.61 vs. 61.90% ; p=0.015), and the associated PF values were 0.49 and 0.59, respectively. But for TC genotype no significant differences were found in patients compared to controls. In contrast, CC genotype and C allele (40.51 vs. 57.69%, P =0.030 respectively) frequencies were significantly increased in patients, compared to controls (23.81 vs. 38.10%; P =0.015), and the associated EF values were 3.29 and 1.58 , respectively. These findings suggest that both IL1-889 and IL-1Rα-maspl 11100 SNP might have a role in the etiopathogenic mechanism of T1DM and IL-1Rα-maspl 11100 polymorphism showed associations (positive and negative) with T1D in the samples of Iraqi patients. Therefore, the functional role of such receptor might have been altered due to the deviations of some genotype and allele frequencies.
Keywords
Polymorphism IL; Ra Diabetes

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