Study the antibacterial Effect of CO2 Laser againstMultidrug Resistant Biofilm formationAcinetobacterbaumanniiIsolated from Different Samples

Natiq Naji, Eman

	Study the antibacterial Effect of CO2 Laser againstMultidrug Resistant Biofilm formationAcinetobacterbaumanniiIsolated from Different Samples
Al-Mustansiriyah Journal for Pharmaceutical Sciences
Article 1, Volume 17, Issue 1, 2017, Pages 148-162
Author
Eman Natiq Naji
Abstract
Acinetobacterbaumanniihas become known as an imperative healthcare associated and multidrug-resistant microorganismwarrants the training of novel methodologies for prevention and treatment. This report aimed to estimate the antimicrobial properties ofCO2laser at 10600 nm on A.baumannii isolates. Two hundred specimens were collected from patients suffering from wound infections, urinary tract infections, respiratory infections and medical equipment samples. 50 samples for each. These samples were gathered from diverse hospitals in BaghdadIraq. The collected specimens were streaked directly on CHROM agar Acinetobacter. The positive culture results were diagnosed genotypicaly, recA gene (a house keeping gene) was used for this purpose. All isolates were tested for antibiotic sensitivity testing and 13 divers of antibiotics were used. Also, the ability of biofilm formation was detected. CO2laser 10600 nm at power densities (1000,1500,2000 and 2500) W/cm2 with exposure time (15,30, 45 and 60) second was used for the irradiation experiment.17 isolates were positive to A. baumannii which were distributed as follows 4 ,6, 3 and 4 isolates from wounds,urine , sputum and medical equipments samples respectively. Antibiotic sensitivitytest results considered ten of A.baumanniias MDR isolates because of its capability to resist ten antibiotics belong to cephalosporins, carbapenems,ampicillin-sulbactam, fluoroquinolones, and aminoglycosides groups. In additions 14(82.35%) out of the 17 isolates were biofilm producer ranged from weak, moderate to strong biofilm produer. Four isolates, one from four different isolation sources were chosen for CO2 laser irradiation each isolate resisted to all antibiotics used in this study and strong biofilm producer. In general, in this study the results showed that when using power densities (2000 and 2500 W/cm2 at exposure time 30s and 45s) of CO2 laser irradiations the maximum rate of killing percentage was ranged from 97% to 100% for A.baumannii isolated from clinical samples while more exposure time were needed (45s and 60s) to get the same killing percentage for A.baumannii isolated from medical equipments. Consequently, the use of CO2 laser 10600 nm which is independent towards the antibacterial agents resistance pattern of MDR A.baumannii could verify advantageous in treatment of various types of infections caused by these bacteria in addition it may be used in sterilization of some medical equipment surfaces.
Keywords
baumannii; Antibacterial effects; Biofilm formation; Laser

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