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A newly modified HPLC method for estimation of dutasteride in prepared niosomes | ||
| Al-Mustansiriyah Journal for Pharmaceutical Sciences | ||
| Article 1, Volume 17, Issue 2, 2017, Pages 104-111 | ||
| Authors | ||
| Nidhal K. Maraie; Mohammed J. Neamah | ||
| Abstract | ||
| A modify, fast, accurate and precise HPLC conditions were developed and validated for the determination of dutasteride (DT) in niosomes derived from proniosomal gel. The work has shown enough isolation for DT from other contents of the niosomes. The isolation of DT was achieved on C18 column (3μm, 125 × 4.6 mm). The mobile phase was a mixture of acetonitrile: water in a ratio of (50:50) and the flow rate was 1.5 mL per minute. The wave length used to detect dutasteride was 210 nm and the ejection volume was 100 μL. The calibration curve of concentrations range from 50 to 0.3 μg/mL obeys Beer-Lambert's law (R² = 1) for DT. The limits of detection were 0.3 µg/mL for DT with high accuracy and precision which approved that no interaction between the drug and other niosomal content unlike the ordinary UV-spectroscopy and the reported HPLC procedure for DT. | ||
| Keywords | ||
| Acetonitrile; Dutasteride; Modified HPLC condition; Niosomes | ||
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