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Molecular identification of Pseudomonas aeruginosa lectins (LecA and LecB) by using polymerase chain reaction | ||
| Journal of University of Anbar for Pure Science | ||
| Article 1, Volume 10, Issue 3, 2016, Pages 1-7 PDF (661.04 K) | ||
| Document Type: Research Paper | ||
| DOI: 10.37652/juaps.2016.134878 | ||
| Authors | ||
| Ahmed N. Hami* 1; Safaa A. Lateef Al Maeny1; Mohamed I. Nader2 | ||
| 1College of Science, University of Al-Anbar | ||
| 2Baghdad University Institute of Genetic Engineering and Biotechnology | ||
| Abstract | ||
| One hundred fifty-two (152) clinical samples included burns, otitis, and wounds samples and were collected from some Baghdad hospitals. About cystic fibrosis samples, 6 samples were obtained from postgraduate students in Institute of Genetic Engineering. Also, 67 environmental samples were collected. After isolation and identification, 83 isolates of Pseudomonas aeruginosa had been identified. Fifty-four (54) isolates, includes 44 clinical isolates and 10 eco isolates, were selected to investigate the Susceptibility Test toward 11 types of antibiotics. 35 isolates were selected for the discovering tests of 16S rRNA , LecA and LecB gene. Indeed, the bacterial isolates exhibited a high resistance against Ampicillin, Amoxicillin and Nalidixic acid; then decreased against Cefotaxime, Ceftazidimim, Tetracycline, Amikacin, Augmantin, Vancomycin, and Chloramphenicol, while the antibiotic Imipenem recorded a low resistance . The results of electrophoresis for PCR productions of gene 16S rRNA, which considered identification gene, showed peaks in the suspected size (956 bp) in all clinical and environmental isolates. The results of electrophoresis for the detection of LecA gene , LecB bands in the size of (369 and 226 base pairs) respectively for all isolates . From another hand, these results showed peaks for LecA and LecB genes with sizes 369 and 226 pb respectively for all clinical and environmental isolates. | ||
| Keywords | ||
| S rRNA | ||
| References | ||
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