Antiviral Activity of leaves extract of lantana camara against the replication of A virus A/Puerto Rico/8/34(PR8)

N. Hasan, R.

	Antiviral Activity of leaves extract of lantana camara against the replication of A virus A/Puerto Rico/8/34(PR8)
Al-Anbar Journal of Veterinary Sciences
Article 1, Volume 10, Issue 1, 2017, Pages 1-8 PDF (451.72 K)
Author
R. N. Hasan
Abstract
Influenza A virus is considered as of the main causative agents of mortality due to the pandemic rate, among those pandemic infection was 1918 in which; the human population lost about 140 million worldwide. In this study, we identified anew anti-viral activity of different concentrations of Lantana camara against the replication of influenza A virus A/Puerto Rico/8/34 (PR8) after the extraction of active compound from the plant leaves using hexane extraction method and testing different concentration on A549, MDCK II cell lines extract on and we checked the effect of this extract on some cytokines like: IFNB and IRF3 using different techniques represented by Luciferase assay. Also, we investigated the effect of this extract on some viral protein expression like NS1 protein using western blotting and real time PCR techniques and we found that the plant extract played important role in restriction of viral replication when we got a low ability of virus to replicate in different cell lines efficiently like the control sample and that was due to the enhancement of activation of cytokine expression like INFB and IRF3 in the cells that were treated with this extract and in the future we can use this extract for such enhancement to resist any viral infection because of its role in regulation of cellular immunity against any viral infection
Keywords
Lantana camara; virus A; Puerto Rico

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