E. Altaai, M., A. Alhadithy, J., K. Al Swediawy, W. (2017). Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.. , 10(3), 47-56. doi: 10.37652/juaps.2016.135141
Mohammed E. Altaai; Jamal A. Alhadithy; Wissam K. Al Swediawy. "Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.". , 10, 3, 2017, 47-56. doi: 10.37652/juaps.2016.135141
E. Altaai, M., A. Alhadithy, J., K. Al Swediawy, W. (2017). 'Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.', , 10(3), pp. 47-56. doi: 10.37652/juaps.2016.135141
E. Altaai, M., A. Alhadithy, J., K. Al Swediawy, W. Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.. , 2017; 10(3): 47-56. doi: 10.37652/juaps.2016.135141

Genetic Diagnosis of tox A and las B Genes in Pseudomonas aeruginosa isolated from different clinical sources.

Journal of University of Anbar for Pure Science
Article 11, Volume 10, Issue 3, 2016, Pages 47-56    PDF (667.99 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2016.135141
Authors
Mohammed E. Altaai* 1; Jamal A. Alhadithy2; Wissam K. Al Swediawy2
1University of Baghdad - Institute of Genetic Engineering and Biotechnology
2University of Anbar - College of Education for Pure Sciences
Abstract
P.aeruginosa bacteria are opportunistic pathogens capable of infection almost every tissue in the body as a result of possessing a large variety of virulence factors which significantly contribute to the pathogenicity of the host events.In this study, the collection of 90 different clinical sample belonging to the sources, for detection the spread of P.aeruginosa in these sources and study some of virulence factors genes possessed by these bacteria. Diagnosed develop isolated after planted on different growth media through phenotypic traits and microscopic examinations, as well as molecular diagnostics definitive diagnosis of isolates that give a positive result as bacteria P.aeruginosa during the previous tests were depending on the gene diagnosis 16SrDNA with a special bacterial sequence of P.aeruginosa. Diagnostic results showed that 47 isolated a 52% belonging for the type of P.aeruginosa that were divided on 15 isolates 31.9% of cases of burns infections, 13 isolates 27.6% of cases of wound infections, 10 isolates 21.3% of cases of middle ear infections, 5 isolates 10.7% of cystic fibrosis, 4 isolates 8.5% of cases of urinary tract infections. It has been investigating the prevalence of tox A gene that encoded to external toxin A (ETA), and las B gene that encoded to elastase enzyme using (PCR) technique, as the results showed the presence of both genes by 100% within the genetic structure of the isolates of P.aeruginosa under study, and despite the higher in all isolates studies cannot be adopted as indicators diagnostic of these bacteria as substitutes for other genes diagnostic because low sensitivity, as well as being the genes of the most prone to mutations.
Keywords
aeruginosa; SrDNA; tox A; lasB
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