A. Awad, B., M. Turkey, A. (2017). Study Gene Expression for phzA Gene for PHzA Gene Responsible of Pyocyanin Production of P. aeruginosaby qPCR.. , 10(3), 62-68. doi: 10.37652/juaps.2016.135143
Bilal A. Awad; Ahmed M. Turkey. "Study Gene Expression for phzA Gene for PHzA Gene Responsible of Pyocyanin Production of P. aeruginosaby qPCR.". , 10, 3, 2017, 62-68. doi: 10.37652/juaps.2016.135143
A. Awad, B., M. Turkey, A. (2017). 'Study Gene Expression for phzA Gene for PHzA Gene Responsible of Pyocyanin Production of P. aeruginosaby qPCR.', , 10(3), pp. 62-68. doi: 10.37652/juaps.2016.135143
A. Awad, B., M. Turkey, A. Study Gene Expression for phzA Gene for PHzA Gene Responsible of Pyocyanin Production of P. aeruginosaby qPCR.. , 2017; 10(3): 62-68. doi: 10.37652/juaps.2016.135143

Study Gene Expression for phzA Gene for PHzA Gene Responsible of Pyocyanin Production of P. aeruginosaby qPCR.

Journal of University of Anbar for Pure Science
Article 13, Volume 10, Issue 3, 2016, Pages 62-68    PDF (489.89 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2016.135143
Authors
Bilal A. Awad* ; Ahmed M. Turkey
University of Anbar - College of Science
Abstract
Identification of 66 bacterial isolates obtained from different sources has been shoud belonging to the bacterium P. aeruginosa in arate of 46 infection isolation and 20 environmental isolates which confirmed by Microscopy diagnosis and Biochemical tests.Micro titration plates method was (MTP) used for detection of isolates abilites to produce Pyocyanin dye. The isolates were differed in their production of Pyocyanin dye between productive with high efficiency, medium efficiency and low efficiency.For the study of gene expression of these isolates, extracted RNA was obtaind from of all isolates, then measure the amount of gene expression by qRT- PCR technology for gene PhzAresponsible for production of Pyocyanin dye, was studied the isolates incuded were 56 as treatment isolates and 10 as control isolates of each gene using the One Step Real time–PCR. It was found that the phzA gene expression by relative Quantification method ,within the range (0.93-2.12) ,with highly significant differences and probability (P<0.001).
Keywords
MTP; Pyocyanin
References
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