Effect of Some Antibiotics in Quarum Sensing Factors of Locally Isolated  Pseudomons aeruginosa

	Effect of Some Antibiotics in Quarum Sensing Factors of Locally Isolated Pseudomons aeruginosa
journal of kerbala university
Article 1, Volume 13, Issue 4, 2017, Pages 239-249 PDF (0 K)
Abstract
Eleven local isolates of P.aeruginosa from patients suffering from urinary tract infection were used, isolates were identified according to morphological and biochemical tests. The identification was confermed by Api 20 E system. Antibiotic susceptibility testing was done for the isolates to determine the most effective against these isolates. Showed that the most effective antibiotic is ciprofloxacin (CIP) and amikacin (AK) and imipenem (IPM) while most of the isolates were resistant to Ampicillin and Tetracycline. The minimum inhibitory concentration for three different antibiotics were study , and showed MIC values of ciprofloxacin (0.25- 64) μg / ml. While MIC values of amikacin (2 -128) μg / ml. While for the imipenem ranging between (2-32) μg / ml. Some associated virulence factors of isolates were studied, it is found that the production of Hemolysin by Pseudomonas aeruginosa as increased by (100)%, all isolates for two types of bacteria produced for the enzyme protease , also gave (100) %, a positive result for the production of biofilm. As the selected isolates show the ability to adhesion on epithelial cell surfaces at a rate between (17-51.66) cell / epithelial cell, as well as bacterial cell surface hydrophobicity test. As for the test swarming movement, the study demonstrated the ability of bacteria to swarm on enriched agar plate (1.5-2.0 ) % agar,as was noted that there is an inverse relationship between the amount of agar and movement swarming fact agar solid hinder the movement of bacteria on the center of nutrient broth . Has also been an effective microbial antibiotics to three in the inhibition of growth and produce virulence factors bacterial isolates . The results indicated that the antibiotics used in the study its effectiveness against isolates studied and graduated effectiveness as follows: Amikacin,Ciprofloxacin and Imipenem also showed antibiotic good effective in inhibiting virulence factors studied .

Statistics Article View: 27 PDF Download: 27