Estimation of Genetic Distances Among Some Local Okra Cultivars Using Randomly Amplified Polymorphic DNA (RAPD) Markers

Khudair Merza, Thamer; Sadaq Jaafer, Hayder

	Estimation of Genetic Distances Among Some Local Okra Cultivars Using Randomly Amplified Polymorphic DNA (RAPD) Markers
Kufa Journal for Agricultural Science
Article 1, Volume 9, Issue 4, 2017, Pages 31-46
Authors
Thamer Khudair Merza; Hayder Sadaq Jaafer
Abstract
An experiment was conducted in Molecular Biological laboratory in the Faculty of Science / University of Kufa in 2016 to find out genetic distance of six Local okra cultivars using Randomly Amplified Polymorphic DNA (RAPD). Polymorphisms were scored within amplified fragment (their number and molecular sizes) using agarose gel electrophoresis after staining with ethidium bromide. Five primers generated polymorphic patterns and selected among studied cultivars. These primers gave 19 Polymorphic bands from total 41 main bands. The results revealed that, the lowest molecular size to the amplified fragments was generated by UBC-514 reach 276 bp while (1654 bp) was amplified by OPX-18 primer that has the highest molecular size of the amplified fragments. From all of the used primers in the experiment, the primer UBC-210 gave the highest values to the main, amplified and polymorphic band numbers which reached 9, 46 and 14 band compared with the lowest values to these bands which reached 0, 12 and 12 band respectively, produced from primer UBC-620. The highest percentage of polymorphism ratio and the primer efficiency from primer UBC-210 reached %64.3 and %0.2 on sequence while, primer UBC-620 did not reveal give any percenage of the polymorphism ratio and primer efficiency. The results also showed that the highest value of genetic dimes ion (genetic distance) was between the two cultivars: 2 Husinawia Karbala and 5 Husinawia Al-Halle that reached 0.676 comparing with the lowest value to the genetic distance between the two cultivars, 3 Batira Baghdad and 5 Husinawia Al-Halle that reached 0.143. Cluster analysis (phylogenetic tree) revealed two main groups (A and B) with 0.533 distance, Cluster A contained 4 cultivars: Batira Baghdad, Khinyasri Al-Basra, Husinawia Al-Halle and Al-Mawassalia while Cluster B contained 2 cultivars: Husinawia Al-Nasirya and Husinawia Karbala. The results concluded that RAPD parameters were important in revealing polymorphism genetic and its efficiency on the primers number and the genetic distance between the studied cultivars.
Keywords
Genetic polymorphism; Genetic tree; Molecular indicators

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