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Study the Immunomodulatory Effect of Ethanolic Neem Seeds Extract on The Immunologic Response in Mice Vaccinated with Proteus vulgaris vaccine | ||
| Jornal of Al-Muthanna for Agricultural Sciences | ||
| Article 1, Volume 3, Issue 1, 2015, Pages 1-13 | ||
| Abstract | ||
| Abstract Abstract This study was carried out to investigate the immunomodulatory effect of the Ethanolic neem seeds extract on the immune response of mice vaccinated with Proteus vulgaris antigens and considered as plant immunomodulators. The study included eight groups; the first group (I) was treated with distilled water. II group was mice treated with Proteus vulgaris antigens only (III): injected subcutaneously with a dose of (600 ug /Kg) ethanolic neem seed extract, IV group was injected subcutaneously with a dose of (400 ug /Kg) ethanolic neem seed extract only, The V, VI groups were treated with combination of p. vulgaris and ethanolic neem seed extract while (VII and VIII) groups were injected with the immunosuppressive drug prednisolone prior to the forthcoming treatment 5 days. All these treatments were carried out at day 1, and then the mice were sacrificed at day 8 to estimate phagocytic activity index by Eliza reader and on day I4 for estimation of lymphocyte transformation by MTT index by Eliza reader and for delayed-type hypersensitivity reaction test at 24 , 48 and 72 hours after proteus vulgaris antigens injection, at day 21 and 28 for (anti-proteus vulgaris antibody titer) by indirect immunoflourescent assay and for serum electrophoresis to estimate the serum fraction. In this regard, all groups of mice showed different significant increases(P≤ 0.01) in the NBT index which represent the phagocytic activity% as compared to group I (0%), which was injected with distilled water (negative control group). The best treatment efficiency was recorded in-group V (57%), while the lowest treatment efficiency was recorded in-group IV (5%). The results of lymphocyte transformation index in mice of all groups showed different significant increases by MTT assay which represent the lymphocyte transformation index as compared to group I (0%). The best treatment efficiency was recorded in-group V (264%), while the lowest treatment efficiency was recorded in-group VI (17%). The anti-Proteus antibodies assessed by indirect immunoflourescent test also showed a significant increase titer in immunomodulator-treated and -vaccinated mice in comparison with negative and positive group At21, 28 days, and the best treatment efficiency was recorded in-group V after 21 days with titer of 512 | ||
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