Activity of some biocontrol agents against Fusarium solani and Macrophomina phaseolina caused cucumber root rot disease in Babylon Province

	Activity of some biocontrol agents against Fusarium solani and Macrophomina phaseolina caused cucumber root rot disease in Babylon Province
Jornal of Al-Muthanna for Agricultural Sciences
Article 1, Volume 2, Issue 2, 2014, Pages 1-22
Abstract
Abstract : This research was aimed to isolation and identification the pathogen of cucumber root rot disease from Babylon fields this pathogen and testing the activity of some Biocontrol agents against it under laboratory and greenhouse conditions. The results were showed the presence of cucumber root rot disease in tested samples from district Babylon region. F.solani and M.phaseolina were identified and revealed more prevalence than other fungal isolates. All tested isolated caused significant reduction to cabbage seed germination percentages compared to control 100 %. F.solani and M.phaseolina isolates also reduced cucumber seed germination (30 and 26.7%) respectively compared to control 100%. Trichoderma harzianum had high antagonistic ability against fungi , the Pseudomonas fluorescens and Bacillus subtilis bacteria which caused 100% inhibition to the growth of examined fungi . highest to antagonistic ability (100%) against pathogens was obtained 10 , 25 and 50% of busturied suspension whereas, the 1% concentration was reduce fungal growth to 82.9 - 87.7%. The results showed that all biocontrol agents used in this experimented revealed significant reduction to the incidence and severity of cucumber root rot under lath house conditions and closed to Beltanol fungicide activity. In other hand, growth of plant (plant height, wet and dry weight) compared to control was treated with bioagent were significant increased. These results appeared that ability of biocontrol agents to cucumber root rot caused by F.solani and M.phaseolina.

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