Isolate saccharomyces boulardii from mangsteen fruits (Garcinia mangostana L.), molecular identify and study its ability to reduce the level of cholesterol and inhibition of E. coli O157:H7 bacteria

	Isolate saccharomyces boulardii from mangsteen fruits (Garcinia mangostana L.), molecular identify and study its ability to reduce the level of cholesterol and inhibition of E. coli O157:H7 bacteria
Jornal of Al-Muthanna for Agricultural Sciences
Article 1, Volume 2, Issue 2, 2014, Pages 1-13
Abstract
Abstract This study was conducted to isolate Saccharomyces boulardii from Mangostin fruits and diagnosed molecularly by using specific primers targeting sequence for the region (ITS) of the 5.8S rRNA gene.As well as studied its ability to reduce the level of cholesterol and inhibition of E. coli O157:H7. The results shown that colonies were with a round shape and colored white to creamy pale when grown at the solid SD and YPD media. They were convex smooth edges, sticky and averaged size of 1-2 mm when grown at 37 °C for 24 hours. Microscopic examine revealed that their cells an oval, or semi-spherical buds sometimes, single or close in clusters. Genomic DNA was isolated from SbR7 Isolate and ITS region of the 5.8S rRNA gene was amplified using PCR.PCR products was sequenced and compared with the sequence of this region in the DNA of S. boulardii available in GenBank (NCBI) using the BLASTn program. Results revealed, this isolate was almost genetically identical (99%) with i standard S. boulardi strains. The ability in cholesterol reduction in modified culture media during 24 and 48 hrs. of incubation is shown by SbR7 and SbC1 isolates and the reduction percentages were 21.69, 23.89% and 53.90, 49.47 % respectively. Isolates SbR7 and SbC2 shown antagonistic activity toward E. coli O 157:117, in which inhibition zone was 18 millimeter for booth.

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