Identification of Cucumber Mosaic Virus (CMV) on eggplant by using some of indicator plants , Immunostrip assay and reverse transcriptase - polymerase chain reaction (RT-PCR)

Abd Alhussein Alfadhal, Fadhal; Sani Swadei Zagier, Shrooq

	Identification of Cucumber Mosaic Virus (CMV) on eggplant by using some of indicator plants , Immunostrip assay and reverse transcriptase - polymerase chain reaction (RT-PCR)
Kufa Journal for Agricultural Science
Article 1, Volume 9, Issue 4, 2017, Pages 92-107
Authors
Fadhal Abd Alhussein Alfadhal; Shrooq Sani Swadei Zagier
Abstract
This study aimed to diagnose cucumber mosaic virus , The cucumber mosaic virus was detected on eggplants by using indicator plants, Immunostrip assay and Reverse Transcriptase - Polymerase Chain Reaction (RT-PCR). Inoculation was done using infected eggplant plants sap on some weed and crop indicator plants including eggplant (Solanum melongena L. ), cucumber (Cucumis sativus L.), tomato (Solanum lycopersicum L.), squash (Cucurbita pepo L.), pumpkin (Cucurbita maxima L.), , cowpea (Vigna unguiculata L.), tobacco (Nicotiana tabacum 'samsun' and Nicotiana rustica) and Nettle-leaved Goose foot (Chenopodium murale L.). Indicator plants test showed that all tested indictors plant were infected with cucumber mosaic virus. Immunostrip assay showed positive results (sedimentation of clear lines) indicating that samples were infected with cucumber mosaic virus for eggplant and indicator plants ( cucumber, tomato and Goose foot plants) compared to no lines at all from healthy plants. cucumber mosaic virus diagnosis was confirmed by RT-PCR as all infected tested plants gave positive results where a Deoxyribo Nucleic Acid of the virus capsid fragment was amplified with CM1 and CM2 primers, the Deoxyribo Nucleic Acid band size was 502 bp compared to healthy plants that gave no band at all. Confirming that the virus used in this study is cucumber mosaic virus.
Keywords
PCR; CMV; Indicator Plants; Immunostrip

Statistics Article View: 113 PDF Download: 100