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Spectrophotometric Determination of Chloramphenicol in Bulk and Pharmaceutical Preparation | ||
| JURNAL OF MADENAT ALELEM COLLEGE | ||
| Article 1, Volume 9, Issue 2, 2017, Pages 114-124 | ||
| Abstract | ||
| Chloramphenicol is spectrophotometrically determined by the formation of a colored charge transfer exhibiting λmax at 445 nm after the reaction of the secondary amine with sodium nitroprusside in the presence of NH2OH in alkaline medium. Classical univariate and chemometric central composite experimental design approaches were used to find the optimum experimental conditions for the parameters affecting the formation of CT-complex. The proposed method is simple and sensitive for the determination of the drug in a concentration rage of 1.0-25.0 μg.mL-1 with molar absorptivity8.142 x 103L.mol-1.cm. and r= 0.9995. The validity of the method was confirmed by finding the regression equation, accuracy, precision, and detection limit. Chloramphenicol was successfully determined in its pharmaceutical preparations by the developed procedure with a reasonable of recovery and precision. Statistical validation of the obtained results was made and the method shows acceptable recovery and repeatability. | ||
| Keywords | ||
| chloramphenicol determination; spectrophotometry; central composite experimental design | ||
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