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In vitro maturation of Local goat oocytes using different concentration of sheep follicular fluid | ||
| Jornal of Al-Muthanna for Agricultural Sciences | ||
| Article 1, Volume 4, Issue 1, 2016, Pages 1-10 | ||
| Abstract | ||
| Abstract This study was conducted by two experiments in the Laboratory of graduate depart of Animal Resources / collage of Agriculture / University of AL muthanna to investigate the effect of Sheep follicular fluid (SFF(with different concentration (0% ,5%, 10%, 15%) to the SMART media as a culture media for In vitro maturation (IVM) After collection from the follicles , the follicular fluid was subjected to inactivation process and supplied with antibiotics (penicillin and streptomycin), then preserved in the Refrigerater under 5c to the time of use The result of IVM shows the concentration 15% SFF was significantly(p<0.05) increase the percent of IVM (59%±0.5) of the Oocyte with cumulus cells compared with the anther concentration 0%,5% and 10% 48 ±0.5, 47 ±0.4 50 ±0.4% Respectively. and non-significantly deference between all concentration of SFF on the percent of IVM of the Oocyte without cumulus cells. | ||
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