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Extraction and Purification of the Urease Enzyme from the Proteus mirabilis Bacteria | ||
| Jornal of Biotechnology Research Center | ||
| Article 1, Volume 11, Issue 2, 2017, Pages 46-51 | ||
| Authors | ||
| Entesar Hussain Ali; Hadeel Hameed Dawud | ||
| Abstract | ||
| Bacterial isolate was identified using vitek diagnosis; it's obtained from burns from Al-imam Ali Hospital which proved its ability to produce Urease by using Luria broth culture media because it is suitable for growing bacterial cells and for extraction of enzyme. Enzyme purification processes include precipitation with Ammonium sulfate, ion exchange chromatography and gel filtration then the enzyme activity was measured for each purification step. The results of Urease purification that extracted from the isolate of Proteus mirabilis using ammonium sulphate showed that the specific activity was 4.71 unit/mg, and the best saturation ratio was (60%), the number of purification times and yield were 3.5and14.6% respectively. Furthermore, the result of purification using Ionic exchange - Cellulose DEAE was gave the specific activity 12.29 units/mg, the number of purification times and the enzymatic yield were 9.2, 8% respectively while the specific activity was 15.1units/mg and the number of purification times and the enzymatic yield 11.27and 14% respectively by using gel filtration. | ||
| Keywords | ||
| Proteus mirabilis; urease; gel; Filtration | ||
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