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COMPREHENSIVE STURY OF IN VITRO FERTILIZATION OF LOCAL GOAT | ||
| The Iraqi Journal of Agricultural Science | ||
| Article 1, Volume 36, Issue 3, 2005, Pages 157-162 | ||
| Abstract | ||
| ABSTRACT Goat ovaries brought from local abattoir within two hour of slaughter at 30° C , oocytes were asprated and then washed in Phosphate Buffer Solution(PBS )for cumulus - oocyte complexes (COCs) removed and these oocytes were divided in to two groups (with and with out cumulus) . Granulated cytoplasm were selected and divided randomly t035 - 40 oocytes in 4-well dishes and incubated for 27 hours at 38.5° C with 5% CO2 atmosphere in air with 95% humidity . In vitro fertilization were performed after maturation oocytes by capacitated fresh sperms taken by artificial vagina from proven fertile bucks , by addition of a microdrops (1 106 sperm) and culturing with matured oocytes for 24 hours at the same environement mentioned above. The large gravian folliculs (g.fs.) had more identified oocytes than the small ones. | ||
| Main Subjects | ||
| Al-Qadisiyah University | ||
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