Molecular Study of the Correlation between Hemophilia and the Thrombophilic Risk Factors in Dohuk Province by using CVD Strip Assay.

Adel Hanoon, Rana

	Molecular Study of the Correlation between Hemophilia and the Thrombophilic Risk Factors in Dohuk Province by using CVD Strip Assay.
Medical Journal of Babylon
Article 1, Volume 14, Issue 4, 2017, Pages 670-675
Author
Rana Adel Hanoon
Abstract
Haemophilia A is an X-linked, recessively inherited bleeding disorder which results from deficiency of procoagulant factor VIII (FVIII). Forty hemophilic patients were enrolled in the current study. Blood sample were collected and DNA was extracted and subjected to multiplex PCR amplification using specific primers which resulted in bands containing all the suspected mutations associated with hemophilia; CVD Strip Assay was also used to differentiate the obtained mutations. The results showed that out of the (40) hemophilic individuals, the higher incidence (55%) was for the MTHFR (A1298C) heterozygotic type of mutation while the lower incidence (5%) for a mutation recorded for factor V (G1691A, leiden), factor V (H1299R, R2), prothrombin (G20210A), and facto XIII (V34L). For the Apo type mutation, B type hasn’t been detected while E type observed in all cases; A1/A2 mutation created the higher incidence with 25% on the other hand, A3/A3 hasn’t been detected and thus it represented 0% of all the Apo mutations
Keywords
Hemophilia; MTHFR; Coagulation factor XIII; CVD Strip Assay

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