Detection of Human Herpesvirus Type-1 Antigen in Tissues of Oral Squamous Cell Carcinoma by Direct Immunofluorecent Assay

Ahmed Mohammed, Najwa; Atiyah Hussein, Areej; Hameed Majeed, Ahlam

	Detection of Human Herpesvirus Type-1 Antigen in Tissues of Oral Squamous Cell Carcinoma by Direct Immunofluorecent Assay
Diyala Journal of Medicine
Article 1, Volume 8, Issue 2, 2015, Pages 38-45
Authors
Najwa Ahmed Mohammed; Areej Atiyah Hussein; Ahlam Hameed Majeed
Abstract
Background: Human herpesvirus is a large enveloped DNA virus and significant human pathogen. Many studies examined oral squamous cell carcinoma for herpes simplex virus and suggested an association with this virus; others have demonstrated human herpesvirus -1 DNA in different part of oral squamous cell carcinoma. Objective: To detect the human herpes virus -1 antigen in tissues of oral squamous cell carcinoma. Patients and methods: Fourty two formalin-fixed, paraffin embedded oral tissues blocks were collected from 30 patients with oral squamous cell and 12 individuals with apparently-healthy oral tissues from archives of histopathology laboratory of college of Dentistry -Baghdad University, during the period from 2010 till 2012. All samples were related to the period between 2004 to 2009. Human herpesvirus -1 antigen was detected by direct immunofluorecent assay (US biological, Cat. No. H2033-08E). Results: Among oral squamous cell carcinoma group, 26 formalin-fixed, paraffin embedded oral tissues blocks were found to contain HHV-1 antigen, this result constituted 86.7% of the total oral squamous cell carcinoma screened for HHV-1 antigen and 75% within apparently-healthy oral tissues. The age of patients ranged from (25-70) years with mean of 53.26 ±12.1years. The highest percentage 60% was diagnosed in the age above 50 years. The percentage in males (61.68%) was more than in females (38.31%). On the other hand there was no significant difference between viral infection, age and gender distribution, while significant correlation noticed with tumor differentiation. Conclusion: The detection of human herpesvirus -1 antigen in oral squamous cell carcinoma and apparently healthy control indicates virus with other factor such as chemicals and radiation, which play important role in the development of oral cancers
Keywords
oral squamous cell carcinoma; human herpesvirus type; immunofluorescence

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