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Using of Polymerase Chain Reaction (PCR) to Confirm the Diagnosis of Listeria monocytogenes and Determination its Antimicrobial Resistanc | ||
| Rafidain Journal of Science | ||
| Article 24, Volume 27, Issue 1, 2018, Pages 17-30 PDF (820.4 K) | ||
| DOI: 10.33899/rjs.2018.141069 | ||
| Authors | ||
| Hiba Kh. Mahmud; Adeeba Y. Shareef | ||
| Abstract | ||
| PCR technique was used for the identification of (14) strains of L.monocytogenes obtained from department of Biology/college of Science/university of Mosul isolated from soil animal barns and grazing areas in Nineveh governorate, that had been identified previously by traditional methods and API Listeria. The presence of the first identification gene hlyA had been detected, (13) strains exhibited positive result, but when PCR used for detection of second identification gene iap, all strains showed positive result.The third identification gene that have been detected was inlA, (11) strains were clear positive, while (3) strains have weak positive results, so (14) strains were positive. The resistance of L.monocytogenes strains was determined to a number of antibiotics: Tetracycline, Trimethoprim and Erythromycin, it was 78.6%, 35.7% and 57.1% respectively. PCR technique was used to detect antibiotic resistance genes like tet(S) for Tetracycline resistance, (2) strains gave positive result, the gene dfrD for Trimethoprim resistance has also detected and (3) strains showed positive result. The presence of ermB for Erythromycin resistance was studied, (2) strains showed positive result. | ||
| Keywords | ||
| monocytogenes; PCR; hlyA; iap; inlA; tet; dfrD; ermB | ||
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