Estimate of The Genetic Diversity For Many Genotypes of Bread Wheat  (Triticum aestivum  L.) Depending on RAPD Technology

A. Hatam Al-karkhi, Hadeel.; M. A. AL-Joburi, Jassim; A. Tahir, Naoroz.

	Estimate of The Genetic Diversity For Many Genotypes of Bread Wheat (Triticum aestivum L.) Depending on RAPD Technology
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 18, Issue 1, 2018, Pages 49-66
Authors
Hadeel. A. Hatam Al-karkhi; Jassim M. A. AL-Joburi; Naoroz. A. Tahir
Abstract
Astudy was conducted at winter season (2014-2015) in Animal Reproduction Labs ./ College of Agriculture / University of Sulaimaniah to estimate the genetic diversity for (17) genotypes of bread wheat (Triticum aestivum L.) depending on molecular levels by method of RAPD (Random Amplified Polymorphic DNA) . planted in plastic pods , after germination the leaves were cut and DNA exitract and pured , then moved on Agaros gel by using 23 randome primeras . The results showed that the 17 of these primers gave number of total bands 1327 resulted from 143 linked location, 128 of these were heterozygous allele (15 Polymorphic bands) were major allels (Monomorphic bands) . The size of multiple bands for all primeras (140-1500 bp) , While the height ralue of polymorphic information content (PIC) was between 0.7756 and 0.9226 refered for increase of ability disting shed for using the primeras . the value of Gene diversity was between 0.8028 and 0.9273 , and these high values has given idea for the range of the ability of these random primeras which used in RAPD levels for detection about genetic variance between the genotypes of wheat depending on presnse of main and hetero zygous frequency of major allele was recorded highly mean from resulted allels from linked location of two primeras OPG-10 and OPA-10 on the DNA of the genotypes (0.3529) , while the least mean was showed (0.1176) for seven of the primeras which were (OPH-11,OPG-08 ,OPG-20 , OPB-18 , OPD-20 , OPG-12 and OPF-03) . These results which were depended in the anlysis of the getic diversity for the genotypes showed thet the biggest genetic diversity was between gehan99 genotype and ZENGIRCI genotype (0.791) , while the least genetic diversity was between two genotypes (TURKMEN and Gerek ) were (0.318) . The studied genotypes were distribued in genetic dendrogram which built dependingon the results of RAPD reactions, showed that these genotypes were dendrogram to three main groups . The RAPD levels showed high ability for distinguished between studied genotypes of wheat.
Keywords
Bread Wheat; Genetic Diversity; RAPD techonology; Dendogram analysis; RAPD

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