Nodes Multiplication, Callus Initiation and Differentiation of  Atropa belladonna L.

N. A. Al-Akaidi, Hiba

	Nodes Multiplication, Callus Initiation and Differentiation of Atropa belladonna L.
Journal of Tikrit University For Agriculture Sciences
Article 1, Volume 18, Issue 1, 2018, Pages 99-112
Author
Hiba N. A. Al-Akaidi
Abstract
Nodes of Atropa belladonna L. produced in vitro cultured on MS medium supplemented with different concentration of BA for multiplication, multiple shoots cultured on MS medium supplemented with different concentrations of IBA or IAA for rooting, also cultured Shoot tips, True leaves, Cotyledon leaves, Hypocotyle and parts of root on MS medium supplemented with different concentrations of 2,4-D for callus initiation and differentiation. Data refers that highest shoots number 7.00 shoot/explant achieved from cultured nodes on MS medium supplemented with 0.75 ml/l BA after 8 weeks, highest shoots number 1.00 shoot/explant achieved from cultured shoot tips on MS medium supplemented with 0.25 ml/l 2,4-D after 8 weeks, highest shoots number 1.00 shoot/explant obtained from True leaves cultured on MS medium supplemented with 0.75 and 1.0 mg/L 2,4-D after 8 weeks while highest shoots number 1.00 shoot/explant achieved from cultured Cotyledon leaves on MS medium supplemented with 0.25 and 0.5 mg/L 2,4-D after 8 weeks while highest shoots number 1.00 shoot/explant achived from cultured Hypocotyle on MS medium supplemented with 0.25 ,0.5 and 0.75 mg/L 2,4-D after 8 weeks. Shoots produced in vitro rooting from cultured on MS medium supplemented with different concentration of IBA or IAA, highest roots number 51.30 root/explant obtained from cultured shoots on MS medium supplemented with 1.5 ml/l IBA after eight weeks, highest fresh weight of callus 2.17 were achived from culture True leaves on MS medium supplemented with 1.00 mg/L 2,4-D after 4 weeks.
Keywords
Belladonna; Nodes; Callus

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