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Evaluation of some Live Probiotic Cells for Decreasing of Blood Lipids Levels in Laboratory Culture Media | ||
| Journal of Tikrit University For Agriculture Sciences | ||
| Article 1, Volume 18, Issue 1, 2018, Pages 222-232 | ||
| Authors | ||
| Nagham N. Alaobady; Amin S. Badawy; Ammer A.Alshak Dhaher | ||
| Abstract | ||
| This study aimed to evaluate the effectiveness of live cells of some Probiotic species which includes Bifidus actiregularis, Lactobacillus rhamnosus GG, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus reuteri, Lactobacillus casei and Saccharomyces boulardii yeast. on the cholesterol levels, Triglycerides (TG), low density lipoprotein (LDL), high-density lipoprotein (HDL) and very low density lipoprotein (VLDL) that cultured on modified media. contained subculture the probiotics strains and prepared a live cells from these probiotics. As well as, the probiotics strains were inoculated to De Man, Rogosa and Sharpe-Plasma media and the Potato dextrose agar-Plasma media was inoculated with Saccharomycis boulardii yeast and incubated for 24 -48 hr. with convenient Temp,The results showed reduction in percentage of cholesterol, triglycerides, LDL and VLDL. However , significant increase in HDL value . The reduce percentages of cholesterol for the treatments of bacterial cells (Lb. acidophilu , Lb. casei , ، Lb. plantarum, Lb. returii، Lb.rhmanosu ,Bifidus actiregularis and Sacch.boulardii ) were 25.00، 27.93، 11.77، 13.23، 17.65، 5.88 و5.97 respectively for 24 hr and incubation periods as 29.41، 30.88، 14.70، 19.11، 22.06، 10.29 و7.45 respectively, The live bacterial cells Lb. acidophilus and Lb. casei showed priority in reducing TG during 24 & 48 hr incubation periods as (28.16 , 25.35 ( and (30.99 , 29.57 ) respectively. However, Bifidus actiregularis showed less decrease in TG as ( 5.63 , 9.86 ) for 24 & 48 hr respectively. Live cells of Lb. acidophilus showed excellence in percentage of HDL which become 40.43% during 24hr , but during 48hr Lb. casei showed priority in increasing HDL (55.33 % ) .Also both bacteria Lb. acidophilus and Lb. casei showed decrease in LDL & VLDL percentage during 24 & 48 hr incubation periods. Live cells of Lb. casei bacteria gave best results in decrease in cholesterol, TG, LDL, and VLDL but increase in HDL percentage comparing with other probiotics used. | ||
| Keywords | ||
| Probiotics; Cholesterol; Triglycerids; Low Density Lipoproteins; Very Low Density Lipoproteins high; density Lipoprotein | ||
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