M. Al-Fahadawi, S., F. Al-Ani, S., G. Yaseen, Y. (2018). Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization. , 11(2), 18-24. doi: 10.37652/juaps.2017.141585
Sabaa M. Al-Fahadawi; Sarab F. Al-Ani; Yusra G. Yaseen. "Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization". , 11, 2, 2018, 18-24. doi: 10.37652/juaps.2017.141585
M. Al-Fahadawi, S., F. Al-Ani, S., G. Yaseen, Y. (2018). 'Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization', , 11(2), pp. 18-24. doi: 10.37652/juaps.2017.141585
M. Al-Fahadawi, S., F. Al-Ani, S., G. Yaseen, Y. Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization. , 2018; 11(2): 18-24. doi: 10.37652/juaps.2017.141585

Detection of genotypes for Giardia lamblia in Iraqi patients feces by using PCR-RFLP techniques based on GDH gene characterization

Journal of University of Anbar for Pure Science
Article 6, Volume 11, Issue 2, 2017, Pages 18-24    PDF (380.64 K)
Document Type: Research Paper
DOI: 10.37652/juaps.2017.141585
Authors
Sabaa M. Al-Fahadawi1; Sarab F. Al-Ani2; Yusra G. Yaseen3
1University of Anbar - Collage of Education for Women
2University of Anbar - Collage of Medicine
3Al-Mustansria University - National centre of Hematology
Abstract
This study was carried out during the period from March 2016 to January 2017 to detect of genotypes and subgenotypes of Giardia lamblia in human feces by PCR-Restriction fragment length polymorphism based on the detection of glutamate dehydrogenase gene and study the corralation between genotype and prescence of symptoms , a total of 101 stool samples were taken from patients(male and female), aged(2-72) years, who suffering from acute or persistent diarrhea and examined by microscope and molecular techniques , the gdh gene was amplified from 19 cases only by a conventional PCR assay by using a specific oligonucleotide sequence for coding region by gdh gene at size 432bp and typed by RFLP analysis, the results showed the total infection with Giardia lamblia was 84(83.1%) according to the results of microscopic examination, of the 19 positive samples, 2(1.98%) were infected with genotype AI , 3(2.97%) genotype AII , 5(4.95%) genotype BIII and 9(8.91%) infected with genotype BIV, with significance differences between the genotype and precence of symptoms
Keywords
Giardia lamblia; GDH gene; PCR; RFLP
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