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Xylanase production by Trichoderma hamatum using Wheat straw and Rice husk as cheap substitution | ||
| Journal Of Wassit For Science & Medicine | ||
| Article 1, Volume 10, Issue 1, 2017, Pages 20-32 | ||
| Abstract | ||
| Abstrac In Iraq, the agricultural wastes represent a significant potential for the development of biorefineries in different sectors such as cereals. Xylanase is a wide spread group of enzyme which can catalyze the endohydrolysis and it has a wide range of application in industrial areas. Trichoderma sp. was selected as a potential producer of xylanase via a solid state fermentation system (SSF) using agri-wasts as substrate. This study aims to produce of Xylanase by Trichoderma hamatum isolated from soil in solid state fermentation (SSF) using wheat straw and rice husks as common and cheap agricultural wastes substrate. Trichoderma hamatum strain was a subculture on (PDA) and then applies to substrate with the addition of 2.0 mL of mineral salt solution. The fermentation conditions were evaluated using parameters such as fermentation days and moisture content. Xylanase production was determined using 1% of xylan as a substrate in the enzyme reaction. The best moisture content and incubation period for xylanase production were determined. The enzyme production of fungus was separately evaluated in two solid substrates (as carbon source) after 5, 6 and 7 days of incubation. 60, 70 and 80% of moisture content effects and cultivation time were tested. Wheat straw was found to be the best natural substrate for high production of xylanase under solid state fermentation compared to submerged fermentation than rice husk. The maximum production of xylanase was after 6 days of incubation was 42.16 U/g where the maximum of production was at 60% of moisture content which was 35.00 U/g in wheat straw. It was discovered that the combination of 60% of moisture content at the sixth day was the high xylanase production in wheat straw , as well as the residue, take place economic for much industrial application as SSF. Keywords: Xylanase, Trichoderma hamatum, rice husk, wheat straw, SSF | ||
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