Hypolipidemic and antioxidant activity of silymarin in streptozotocin-induced diabetic male rats

PhD,; Aidi,; A.A.Al-Sa, Jabbar; Professor,; BVMS,

	Hypolipidemic and antioxidant activity of silymarin in streptozotocin-induced diabetic male rats
Almuthanna Journal of Pure Science (MJPS)
Article 1, Volume 2, Issue 1, 2014, Pages 0-0
Authors
; ; Jabbar A.A.Al-Sa; ;
Abstract
The potency of silymarin in ameliorating the lipid peroxidation and antioxidant status has been evaluated in streptozotocin-induced diabetic rats. After induction of diabetes, by single injection of streptozotocin (60 mg/ kg bw), ninety six (48 intact and 48 diabetic) mature male rats were assigned to 4 equal groups, treated for three periods (15, 25, and 35 days) by drenching intact and diabetic rats with drinking water (C and D groups) and drenching intact and diabetic rats with 200 mg/kg bw of silymarin suspension (SD and S groups). Body weights has been registered at five days interval. After the last day of each period, 8 males from each group were anaesthetized, sacrificed, and blood samples were obtained for assessment of serum lipid profile (total cholesterol; T-c, triglycerides; TG, high density lipoprotein; HDL-c, low density lipoprotein; LDL-c, and very low density lipoprotein; VLDL-c), liver function test (ALT, AST, and ALP concentrations), and oxidant- antioxidants activity ( superoxide dismutase; SOD, catalase; CAT, total glutathione; GSH, glutathione reductase; GSH-r, glutathione transferase; GTH-t, glutathione peroxidase; GSH-px and malondialdehyde; MDA). Body weight showed marked increase in control and silymarin treated male rats compared with diabetic control in all periods of experiment. Serum ALT, AST, ALP, T-c, LDL-c, and VLDL-c concentrations decreased significantly in C, DS, and S groups compared with diabetic control (D group) at the end of each period of the experiment, whereas TG and HDL concentration showed significant increase at the same periods. At the end of each period of the experiment, serum oxidant-antioxidant status revealed significant decrease of serum MDA, SOD, CAT, GSH-t, GSH-r and GSH-px activity, whereas total GSH activity was significantly increased. It can be concluded that 200 mg/kg b.w. of silymarin drenching for 15, 25, and 35 days perform positive role in ameliorating the different complications of oxidative stress produced by diabetes mellitus.

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