Inhibition of Binding of Helicobacter pylori to Tissue Culture Cells by Anti-Lipopolysaccharides Monoclonal Antibodies

MaanAbul-Milh,

	Inhibition of Binding of Helicobacter pylori to Tissue Culture Cells by Anti-Lipopolysaccharides Monoclonal Antibodies
Almuthanna Journal of Pure Science (MJPS)
Article 1, Volume 2, Issue 1, 2014, Pages 0-0
Author

Abstract
Binding of 35[S]- methionine-labeled Helicobacter pylori to tissue culture was carried out using human colon carcinoma epithelial cells (Caco-2), and human adenocarcinoma tissue culture cells (AGS). In this research, clinical isolates (smooth) as well as type culture collection (rough) strains were used in binding and inhibition of the binding study of H. pylori to tissue cultures using monoclonal antibodies (MAbs) raised against bacterial lipopolysaccharides (LPS). The task of the work was to discriminate and evaluate the role of LPS as bacterial adhesin in the binding as well as the specificity of the binding of various H. pylori strains to cell lines. Binding of H. pylori, clinical isolate Hel 305(smooth strain),to Caco-2 cells was strongly inhibitedby species-specific MAbs raised against Hel 305-LPS antigen. It was also recognized that inhibition of binding of Hel 305 strain to Caco-2 cells was high effective when using species-specific MAbs in comparison with MAbs raised against LPS from type culture collection CCUG 17874 (rough strain). In the same manner, binding of rough CCUG 17874 strain to Caco-2 cell line was inhibited by species-specific LPS MAbs stronger than with MAbs raised against Hel 305 LPS. In both overlay cases, no significant inhibition with other LPS MAbs of different origins was detected, after bacterial incubation with MAbs raised against LPSs from other tested H. pylori strains.

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