Preparation of Vaccine for pathogenic bacteria causing abortion using low level diode laser

Ihsan, Prof.Dr.; Hedaa,; Rostum, F.; Nahaab, M.

	Preparation of Vaccine for pathogenic bacteria causing abortion using low level diode laser
Almuthanna Journal of Pure Science (MJPS)
Article 1, Volume 1, Issue 1, 2013, Pages 0-0
Authors
Prof.Dr. Ihsan; ; F. Rostum; M. Nahaab
Abstract
The objective of this study was investigate the effect of Low Level diode laser on the bacterial isolates (Listeria monocytogenes, Enterobacter cloacae, Klebsiella pneumonia, Staph aureus, Esherichia coli and Pseudomonas aeruginosa) which used in preparation of vaccine and to determine the immune responses using the RID kit. The antibacterial agent’s sensitivity before irradiation was performed by using twelve antibiotics, these strains showed high resistance for these antibiotics except amikacin. But after irradiation the sensitivity was increased. Some features were studied such as bacterial count which decreased and lost of blood hemolysis and biocyanine stain from p.aeruginosa according to increasing the time and power of irradiation. Bacterial growth killed and attenuated to prepare vaccine using diode laser (660) nm of wavelength having (50 and 250) mw of power and the frequency (1-10) kHz. The experiment was conducted on 24 adult white New Zealand male rabbits with (1.5-2) Kg body weight each, they were divided into sixth groups with 4 rabbits each and inoculated with killed and attenuated vaccine and one group was used as control. After (35day) of immunization, determination was done for (IgM, IgG, IgA in addition to C3 and C4). The Ig and Co. of immunized animals were higher (P>0.05) compared with control animals, also the live attenuated vaccine induced highly immune response as compared with killed vaccine. The control group died after challenge dose while the immunized animals not.
Keywords
Key Words; bacteria; Vaccine; Laser

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