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Molecular investigation of the Parasite Entamoeba Spp. among children with diarrhea in Thi Qar Province | ||
| Journal of Univesity of Thi-Qar | ||
| Article 1, Volume 12, Issue 4, 2017, Pages 16-30 | ||
| Authors | ||
| Fadhil Abbas Manshad; Manar Kareem Kadhm | ||
| Abstract | ||
| The purpose of the current study is the genetic diagnosis of the genus Entamoeba Using PCR technology and identify some of the species that infect humans using Nested PCR by 18S rRNA gene responsible for this genus discrimination. The current study included 300 Sample microscopically examined for a period for October 2015 to Septembre 2016 - turned out the presence total infection rate for Entamoba complex 26.86%. Eighty samples of microscopically positive were examined moleculary and affirmed the presence 35% of Entamoba spp. When Nested PCR examination was used to determine the species (Species Specific) the infection rate 39.29% appeared to Entamoeba histolytica and 39.29% for Entamoeba. The results of this study refer that the Nested PCR technique was efficiency in distinguishing and identifying species of the parasite with high accuracy and reliability, high-speed compared to microscopic examination is able to distinguish the species of Entamoeba and The high sensitivity of molecular ways help to reach the true prevalence of this genus and We can use these molecular methods in epidemiological studies. | ||
| Keywords | ||
| PCR; Nested PCR | ||
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